Other

Part:BBa_K5452108:Design

Designed by: Bianca Henriques de Oliveira   Group: iGEM24_USP-EEL-Brazil   (2024-09-28)


Untranslated regions (3’ UTR) located in the hexocinase locus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 268
    Illegal XbaI site found at 617
    Illegal SpeI site found at 793
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 268
    Illegal SpeI site found at 793
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 268
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 268
    Illegal XbaI site found at 617
    Illegal SpeI site found at 793
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 268
    Illegal XbaI site found at 617
    Illegal SpeI site found at 793
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose the hexokinase gene because its knockout does not interfere with yeast growth. Deletion of the HXK isoenzyme can trigger an increase in the expression of another HXK, causing an increase in the flow of the glycolytic pathway (Sun et al., 2014).



Source

This part can be found in the genome of the Saccharomyces cerevisiae

References